Services

The following unique core facilities are available on-site:

NMR

• Bruker Avance 500MHz NMR equipped with several probes including a 5mm Z gradient BBO probe (mainly for 13C spectra), and 5mm, 1.7mm, and 1mm Z-gradient TXI probes (for 1H and 13C spectra). This instrument can generate 1D as well as other multidimensional spectra such as COSY, TOCSY, HSQC, HMBC, and HSQC-TOCSY.

LCMS

• 1 single-quadrapole Waters Micromass ZQ ESI spectrometer coupled to an Agilent 1100 HPLC
• 1 single-quadrapole Waters Acquity QDa ESI Mass Detector coupled to a Waters I-Class UPLC
• 1 Sciex triple quad/ linear ion trap QTrap 4000 (for quantitation and MSn experiments) coupled to a Sciex Exion HPLC
• 1 Waters G2XS qTOF mass spectrometer coupled to a Waters I-Class UPLC for high resolution mass spectrometry and metabolomics
• 1 Mass Lynx / Progenesis Work Station

Other Core Facilities Available

• Microscope Model: Zeiss Axio Imager.Z1.
  • S/N: 3512000350.
  • Settings: brightfield, darkfield, phase contrast, differential interference contrast (DIC), fluorescence.
  • Objectives: 2.5x, 20x, 40x, 40x (oil), 100x (oil).
  • Cameras: AxioCam HRm and AxioCam MRc5
• BD FACSCelesta Flow Cytometer with 12-color detection
  • blue (488), violet (405), red (640) laser configuration (excitation wavelengths)
  • blue filters: 530/30, 575/26, 610/20, 695/40
  • violet filters: 450/40, 525/50, 610/20, 660/20, 780/60
  • red filters: 670/30, 730/45, 780/60
  • Configured for both single tube and high throughput (HTS module) for 96-well or 384-well analysis
  • BD FACSDiva software

Other Unique Core Facilities Available Through Agreement

• Standard DNA sequencing and fragment analysis
  • The Core has an Applied Biosystems 3130xl Genetic Analyzer that is used for standard DNA sequencing and DNA fragment sizing analyses. Instrument ready reactions can be loaded and run by trained and approved personnel.
• Next Generation DNA sequencing
  • The Core has a Roche GS Junior System that is capable of sequencing complete prokaryotic genomes and eukaryotic transcriptomes.
• Real-time PCR
  • The Core has an Applied Biosystems 7500 Real-Time PCR instrument. Instrument ready reactions can be loaded and run by trained and approved personnel.
• The Nutrient Analysis laboratory houses six main instruments
  • 4 channel Bran+Luebbe AutoAnalyzer III, Shimadzu TOC 5050 and Antek 9000N, CE Elantech NC2100, Turner 10AU fluorometer, Shimadzu UV 1601 Spectrophotometer

Building features include:

• LEED Silver certification
• 24/7 physical and IT security
• Full power backup protection/100% power back-up
• Designed to withstand Category 3 hurricane force winds

The open floorplans and modular design for lab space provide tenants with a wide range of options to accommodate diverse workflows and research needs.

Lab features include:

• Contiguous office and labs
• Modular space (infinite configurations without construction)
• Movable lab tables
• Glassware washers and dryers
• A/V screen and ceiling-mounted projector
• White boards
• Extra equipment storage space
• Wall-mounted hoods and sinks
• Ceiling-mounted utilities

1. Affordable, modular laboratories for rent designed by scientists - save time!
2. Attractive coastal lifestyle with three local island beaches
3. Supportive environment for start-up, growing and established organizations
4. Business-minded and collaborative research community
5. Healthy and eco-friendly Silver LEED certified facility
6. On-site core resources
7. Lower cost labor pool located in Southeastern North Carolina - save money!
8. Affordable housing in all market sectors
9. Marine-themed cafeteria and welcoming social spaces
10. Natural wooded environment located directly on the Atlantic Ocean

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